2017-8-11 16:0:32025-05-25 04:48:37
description
trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component of the PTS
sucrose permease of the [SW|phosphotransferase systems|phosphotransferase system], EIIBC of the [[category|Phosphotransferase system|PTS]], [[category|Trigger enzyme]], control of [[protein|SacT]] activity
locus
BSU38050
BSU_38050
geneLength
1383
1386
function
sucrose uptake and phosphorylation, control of SacT activity
sucrose uptake and phosphorylation, control of [[protein|SacT]] activity
product
trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component
sucrose-specific [[category|Phosphotransferase system|PTS]] permease, EIIBC component
outlinks
bsu
BSU38050
BSU_38050
Gene
Coordinates
3,903,646 → 3,905,031
3,903,646 3,905,031
The protein
Catalyzed reaction/ biological activity
Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)
The protein
Protein family
[[protein|PtsI]] permease, sucrose permease (Scr) family [Pubmed|10627040]
[[category|Phosphotransferase system|PTS]] permease, sucrose family [Pubmed|10627040]
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
cell membrane (according to UniProt)
Biological materials
Expression vector
for expression, purification of the EIIB domain in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP429, available in [SW|Stülke] lab
for expression, purification of the EIIB domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP436, available in [SW|Stülke] lab
References
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|PTS EIIB domain] type-1 (aa 1-87) (according to UniProt)
[SW|PTS EIIC domain] type-1 (aa 107-461) (according to UniProt)
Biological materials
Mutant
BKE38050 ([[gene|sacP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTTCTCCCCCTTTT, downstream forward: _UP4_AATGAGGATGAGGAGAGGAA
BKK38050 ([[gene|sacP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTTCTCCCCCTTTT, downstream forward: _UP4_AATGAGGATGAGGAGAGGAA
Biological materials
Expression vectors
for expression, purification of the EIIB domain in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP429, available in [SW|Jörg Stülke]'s lab
for expression, purification of the EIIB domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP436, available in [SW|Jörg Stülke]'s lab